การตรวจนับจุลชีพในตัวอย่างของเหลว Membrane filter method

Commonly tested parameters ( Water Analysis )

Sample Preparation

For indicator organisms (AQ, EC, ETC, and PA):

For samples with very low microbial load (e.g potable water)

- Filter 100ml of liquid sample using a 47mm membrane filter with 0.45µ pore size.

For samples with low to high microbial load (e.g surface water and waste water)

- Directly inoculate 1ml of sample or if suspected to be high, dilute to 10-1 using sterile diluent.

For Pathogen (SL):

Direct – Measure 100ml water sample and dilute with 100ml sterile double strength Buffered Peptone Water (BPW).

Filtration – Filter 100ml water sample and transfer filter to 100ml sterile BPW.

Regulatory Limits

Refer to Word Health Organization (WHO), Environmental Protection Agency (EPA), or other local regulatory bodies.